NEFL Monoclonal Antibody

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Home > Product > Antibody > NEFL Monoclonal Antibody

NEFL Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA3662

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human NEFL (AA: 1-200) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Uniprot accession

P07196:;

Molecular weight

61.5kDa

Gene ID

4747

Synonyms

NFL;NF-L;NF68;CMT1F;CMT2E;CMTDIG;PPP1R110

Research area

Neuroscience

Target protein

NEFL

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using NEFL mAb against human NEFL (AA: 1-200) recombinant protein. (Expected MW is 26.9 kDa)

Western blot analysis using NEFL mAb against HEK293-6e (1) and NEFL (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

Flow cytometric analysis of SK-N-SH cells using NEFL mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NEFL mouse mAb with DAB staining.

Western blot analysis using NEFL mouse mAb against HEK293 (1) cell lysate.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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