CD47 Monoclonal Antibody

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Home > Product > Antibody > CD47 Monoclonal Antibody

CD47 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4114

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD47 (AA: 19-141) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot accession

Q08722:;

Molecular weight

35.2kDa

Gene ID

961

Synonyms

IAP;OA3;MER6

Research area

Immunology

Target protein

CD47

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD47 mAb against human CD47 (AA: 19-141) recombinant protein. (Expected MW is 16.8 kDa)

Western blot analysis using CD47 mAb against HEK293-6e (1) and CD47 (AA:19-141)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Hela cells using CD47 mouse mAb (green) and negative control (red).

Flow cytometric analysis of Jurkat cells using CD47 mouse mAb (green) and negative control (red).

Flow cytometric analysis of K562 cells using CD47 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD47 mouse mAb with DAB staining.

Western blot analysis using CD47 mouse mAb against Jurkat (1), MOLT4 (2), HL-60 (3), Raji (4), Ramos (5), and HEK293 (6) cell lysate.

Immunofluorescence analysis of Hela cells using CD47 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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