CD62E Monoclonal Antibody

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Home > Product > Antibody > CD62E Monoclonal Antibody

CD62E Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: ICC,FCM

Product overview

Code

BT-MCA3991

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD62E (AA: extra(22-162)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot accession

P16581:;

Molecular weight

66.7kDa

Gene ID

6401

Synonyms

ELAM;ESEL;SELE;ELAM1;LECAM2

Research area

Others

Target protein

CD62E

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD62E mAb against human CD62E (AA: extra(22-162)) recombinant protein. (Expected MW is 42.3 kDa)

Western blot analysis using CD62E mAb against HEK293-6e (1) and CD62E (AA: extra(22-162))-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Jurkat cells using CD62E mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using CD62E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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