CD3D Monoclonal Antibody

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Home > Product > Antibody > CD3D Monoclonal Antibody

CD3D Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: ICC,FCM

Product overview

Code

BT-MCA2004

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Uniprot accession

P04234:;

Molecular weight

18.9kDa

Gene ID

915

Synonyms

T3D;IMD19;CD3-DELTA

Research area

Immunology

Target protein

CD3D

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200-1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western blot analysis using CD3D mAb against human CD3D (AA: 127-171) recombinant protein. (Expected MW is 18.6 kDa)

Western blot analysis using CD3D mAb against HEK293-6e (1) and CD3D (AA: 127-171))-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Jurkat cells using CD3D mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using CD3D mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using CD3D mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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