CD99 Monoclonal Antibody

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Home > Product > Antibody > CD99 Monoclonal Antibody

CD99 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2233

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Uniprot accession

P14209:;

Molecular weight

18.8kDa

Gene ID

4267

Synonyms

MIC2;HBA71;MIC2X;MIC2Y;MSK5X

Research area

Immunology

Target protein

CD99

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200-1:1000
ICC	1:200 - 1:1000
FCM	1:200-1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western blot analysis using CD99 mAb against human CD99 (AA: 1-185) recombinant protein. (Expected MW is 43 kDa)

Western blot analysis using CD99 mAb against HEK293-6E (1) and CD99 (AA:1-185)-hIgGFc transfected HEK293-6E (2) cell lysate.

Flow cytometric analysis of Jurkat cells using CD99 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded ewing sarcoma tissues using CD99 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD99 mouse mAb with DAB staining.

Western blot analysis using CD99 mouse mAb against THP-1 (1), U937 (2), and MOLT4 (3) cell lysate.

Immunofluorescence analysis of Hela cells using CD99 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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