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Home > Product > Antibody > MDM2 Monoclonal Antibody

MDM2 Monoclonal Antibody

MDM2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: FCM

Product overview

Code

BT-MCA4700

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MDM2 (AA: 26-169) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]

Uniprot accession

Molecular weight

55.2kDa

Gene ID

4193

Synonyms

HDMX;LSKB;hdm2;ACTFS

Research area

Cell Biology

Target protein

MDM2

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
FCM 1:200-1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using MDM2 mAb against human MDM2 (AA: 26-169) recombinant protein. (Expected MW is 19.4 kDa)
Western blot analysis using MDM2 mAb against HEK293 (1) and MDM2 (AA:26-169)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric analysis of Hela cells using MDM2 mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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