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Home > Product > Antibody > SP17 Monoclonal Antibody

SP17 Monoclonal Antibody

SP17 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,FCM

Product overview

Code

BT-MCA4439

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SP17 (AA: 1-152) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]

Uniprot accession

Molecular weight

17.4 kDa

Gene ID

53340

Synonyms

CT22;SPA17;SP17-1

Research area

Others

Target protein

SP17

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200-1:1000
FCM 1:200-1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)
Western blot analysis using SP17 mAb against HEK293-6e (1) and SP17 (AA: 1-152)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using SP17 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using SP17 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded testis tissues using SP17 mouse mAb with DAB staining.

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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