POLR2A Monoclonal Antibody

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Home > Product > Antibody > POLR2A Monoclonal Antibody

POLR2A Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: FCM

Product overview

Code

BT-MCA4834

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human POLR2A (AA: 128-258) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Uniprot accession

P24928:;

Molecular weight

217kDa

Gene ID

5430

Synonyms

RPB1;RPO2;POLR2;POLRA;RPBh1;RPOL2;RpIILS;hsRPB1;hRPB220

Research area

Epigenetics and Nuclear Signaling

Target protein

POLR2A

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using POLR2A mAb against human POLR2A (AA: 128-258) recombinant protein. (Expected MW is 41.1 kDa)

Western blot analysis using POLR2A mAb against HEK293 (1) and POLR2A (AA: 128-258)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric analysis of Hela cells using POLR2A mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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