EHMT2 Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > EHMT2 Monoclonal Antibody

EHMT2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA3202

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.

Uniprot accession

Q96KQ7:;

Molecular weight

132.4kDa

Gene ID

10919

Synonyms

G9A;BAT8;GAT8;NG36;KMT1C;C6orf30

Research area

Developmental biology,Epigenetics and nuclear signaling,Stem cells

Target protein

EHMT2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using EHMT2 mAb against human EHMT2 (AA: 317-471) recombinant protein. (Expected MW is 44 kDa)

Western blot analysis using EHMT2 mAb against HEK293 (1) and EHMT2 (AA: 317-471)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis of Hela cells using EHMT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HL-60 cells using EHMT2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using EHMT2 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EHMT2 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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