PCK2 Monoclonal Antibody

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Home > Product > Antibody > PCK2 Monoclonal Antibody

PCK2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3818

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PCK2 (AA: 44-175) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

Uniprot accession

Q16822:;

Molecular weight

70.7kDa

Gene ID

5106

Synonyms

PEPCK;PEPCK2;PEPCK-M

Research area

Signal transduction,Cancer,Metabolism,Cardiovascular

Target protein

PCK2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using PCK2 mAb against human PCK2 (AA: 44-175) recombinant protein. (Expected MW is 40.5 kDa)

Western blot analysis using PCK2 mAb against HEK293 (1) and PCK2 (AA: 44-175)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using PCK2 mouse mAb against Jurkat (1), C2C12 (2), Hela (3), HepG2 (4), COS7 (5), and HL-60 (6) cell lysate.

Immunofluorescence analysis of Hela cells using PCK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using PCK2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PCK2 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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