ATG4A Monoclonal Antibody

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Home > Product > Antibody > ATG4A Monoclonal Antibody

ATG4A Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: ICC,FCM

Product overview

Code

BT-MCA3279

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ATG4A (AA: 258-398) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Uniprot accession

Q8WYN0:;

Molecular weight

45.4kDa

Gene ID

115201

Synonyms

APG4A;AUTL2

Research area

Cancer

Target protein

ATG4A

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:100 - 1:400
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using ATG4A mAb against human ATG4A (AA: 258-398) recombinant protein. (Expected MW is 42.2 kDa)

Western blot analysis using ATG4A mAb against HEK293 (1) and ATG4A (AA: 258-398)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of Hela cells using ATG4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HL-60 cells using ATG4A mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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