TRIM25 Monoclonal Antibody

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Home > Product > Antibody > TRIM25 Monoclonal Antibody

TRIM25 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3403

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Uniprot accession

Q14258:;

Molecular weight

71kDa

Gene ID

7706

Synonyms

EFP;Z147;RNF147;ZNF147

Research area

Cell Biology

Target protein

TRIM25

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:50 - 1:250
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)

Western blot analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.

Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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