PLD2 Monoclonal Antibody

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Home > Product > Antibody > PLD2 Monoclonal Antibody

PLD2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA3506

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PLD2 (AA: 834-933) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

O14939:;

Molecular weight

106kDa

Gene ID

5338

Synonyms

Research area

Cardiovascular

Target protein

PLD2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)

Western blot analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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