MGMT Monoclonal Antibody

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Home > Product > Antibody > MGMT Monoclonal Antibody

MGMT Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2745

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Uniprot accession

P16455:;

Molecular weight

21.6kDa

Gene ID

4255

Synonyms

EC 2.1.1.63

Research area

Epigenetics and Nuclear Signaling

Target protein

MGMT

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)

Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.

Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MGMT mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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