CSNK2B Monoclonal Antibody

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Home > Product > Antibody > CSNK2B Monoclonal Antibody

CSNK2B Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3811

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CSNK2B (AA: FULL(1-215)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P67870:;

Molecular weight

25kDa

Gene ID

1460

Synonyms

G5A;CK2B;CK2N;CSK2B

Research area

Cell Biology

Target protein

CSNK2B

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using CSNK2B mAb against human CSNK2B (AA: FULL(1-215)) recombinant protein. (Expected MW is 35 kDa)

Western blot analysis using CSNK2B mAb against HEK293 (1) and CSNK2B (AA: FULL(1-215))-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), K562 (3), HepG2 (4), C6 (5), SK-N-SH (6), NTERA-2 (7), MCF-7 (8), NIH/3T3 (9) cell lysate.

Immunofluorescence analysis of MCF-7 cells using CSNK2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using CSNK2B mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CSNK2B mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CSNK2B mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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