PDK2 Monoclonal Antibody

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Home > Product > Antibody > PDK2 Monoclonal Antibody

PDK2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA4545

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Uniprot accession

Q15119:;

Molecular weight

46.2kDa

Gene ID

5164

Synonyms

PDHK2;PDKII

Research area

Signal Transduction,Metabolism

Target protein

PDK2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using PDK2 mAb against human PDK2 (AA: 178-404) recombinant protein. (Expected MW is 51.2 kDa)

Western blot analysis using PDK2 mAb against HEK293 (1) and PDK2 (AA: 178-404)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of Hela cells using PDK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using PDK2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PDK2 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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