EIF2AK2 Monoclonal Antibody

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Home > Product > Antibody > EIF2AK2 Monoclonal Antibody

EIF2AK2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4033

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Uniprot accession

P19525:;

Molecular weight

62kDa

Gene ID

5610

Synonyms

PKR;PRKR;EIF2AK1

Research area

Signal Transduction

Target protein

EIF2AK2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)

Western blot analysis using EIF2AK2 mAb against HEK293 (1) and EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.

Flow cytometric analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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