SQSTM1 Monoclonal Antibody

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Blood coagulation
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Epigenetics and nuclear signaling
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Home > Product > Antibody > SQSTM1 Monoclonal Antibody

SQSTM1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4761

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SQSTM1 (AA: 232-356) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Uniprot accession

Q13501:;

Molecular weight

47.7kDa

Gene ID

8878

Synonyms

p60;p62;A170;OSIL;PDB3;ZIP3;p62B

Research area

Epigenetics and nuclear signaling,Cancer,Signal transduction,Cardiovascular,Metabolism

Target protein

SQSTM1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)

Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.

Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SQSTM1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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