14-3-3 Sigma Polyclonal Antibody

Product overview

Full product name

14-3-3 sigma Antibody

Code

BT-AP00014

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SFN. AA range:41-90

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

SFN (Stratifin) is a Protein Coding gene. Diseases associated with SFN include pancreatic ductal adenocarcinoma and limbal stem cell deficiency. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include protein kinase binding and phosphoprotein binding. An important paralog of this gene is YWHAZ. dapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. 53-regulated inhibitor of G2/M progression.

Uniprot accession

Human:;Mouse:;

Molecular weight

27774

Gene ID

Human: 2810; Mouse: 55948

Synonyms

SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin

Research area

Neuroscience; Signal Transduction

Target protein

SFN

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	100 - 300
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of lysates from HT29 cells and RAW264.7 cells, using 14-3-3 sigma Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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