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Home > Product > Antibody > Caspase-6 Polyclonal Antibody

Caspase-6 Polyclonal Antibody

Caspase-6 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Caspase-6 Antibody

Code

BT-AP01205

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:223-272

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CASP6 encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein (caspase 6) is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of CASP6 results in multiple transcript variants that encode different isoforms.

Uniprot accession

Molecular weight

33310

Gene ID

Human: 839; Mouse: 12368

Synonyms

CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2

Research area

Neuroscience

Target protein

CASP6

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 20000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Caspase 6 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western Blot analysis of various cells using Caspase-6 Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from HepG2 and RAW264.7 cells, using Caspase 6 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using Caspase 6 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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