CD34 Polyclonal Antibody

Product overview

Full product name

CD34 Antibody

Code

BT-AP01486

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CD34. AA range:208-257

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CD34 molecule encoded by CD34 may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for CD34.

Uniprot accession

Human:;Mouse:;

Molecular weight

40716

Gene ID

Human: 947; Mouse: 12490

Synonyms

CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Research area

Neuroscience

Target protein

CD34

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
ELISA	1: 10000
IHC-p	100 - 300

Images

Western Blot analysis of various cells using CD34 Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of lysate from HepG2 cells, using CD34 antibody.

Western Blot analysis of HepG2 cells using CD34 Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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