Immunofluorescence analysis of rat-spleen tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-kidney tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western Blot analysis of various cells using c-Fos Polyclonal Antibody diluted at 1:2000 cells nucleus.
Immunofluorescence analysis of Hela cell. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-SMA Monoclonal Antibody(6A12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Immunofluorescence analysis of rat-lung tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,c-Fos Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-kidney tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of the lysates from HepG2 cells using Fos antibody.
Immunofluorescence analysis of rat-lung tissue. 1,c-Fos Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western Blot analysis of 293T cells using c-Fos Polyclonal Antibody diluted at 1:2000 cells nucleus.
Western blot analysis of lysates from HeLa cells, using Fos Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using c-Fos Polyclonal Antibody diluted at 1:500 cells nucleus.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Fos Antibody. The picture on the right is blocked with the synthesized peptide.
