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Home > Product > Antibody > Chk2 Polyclonal Antibody

Chk2 Polyclonal Antibody

Chk2 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

Chk2 Antibody

Code

BT-AP01769

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CHEK2. AA range:35-84

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The checkpoint kinase 2 encoded by CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Molecular weight

60915

Gene ID

Human: 11200; Mouse: 50883

Synonyms

CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Research area

Epigenetics and Nuclear Signaling

Target protein

CHEK2

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
ELISA 1: 10000

Images

Western Blot analysis of various cells using Chk2 Polyclonal Antibody diluted at 1:500 cells nucleus.
Western Blot analysis of HuvEc cells using Chk2 Polyclonal Antibody diluted at 1:500 cells nucleus.
Western blot analysis of Chk2 Polyclonal Antibody, using Hela, HCT116,SW480, HEK293 cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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