CHOP Polyclonal Antibody

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Home > Product > Antibody > CHOP Polyclonal Antibody

CHOP Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

CHOP Antibody

Code

BT-AP01775

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CHOP. AA range:15-64

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

DDIT3 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. DNA damage inducible transcript 3 functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Uniprot accession

Human:;Mouse:;

Molecular weight

19175

Gene ID

Human: 1649; Mouse: 13198

Synonyms

DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GA

Research area

Epigenetics and Nuclear Signaling

Target protein

DDIT3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 5000

Images

Western Blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1:500

Western Blot analysis of VEC cells using CHOP Polyclonal Antibody diluted at 1:500

Immunofluorescence analysis of HeLa cells, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Western blot analysis of lysates from HeLa, Jurkat, and COLO205 cells, using CHOP Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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