Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp315) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western Blot analysis of various cells using Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp315) Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of 293 cells using Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
