Creatine Kinase M Polyclonal Antibody

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Home > Product > Antibody > Creatine Kinase M Polyclonal Antibody

Creatine Kinase M Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Creatine Kinase M Antibody

Code

BT-AP02207

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human M-CK. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Creatine kinase, M-typeencoded by CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

43101

Gene ID

Human: 1158; Mouse: 12715; Rat: 24265

Synonyms

CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK

Research area

Signal Transduction; Metabolism

Target protein

CKM

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HepG2 cells, using M-CK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using M-CK antibody.

Western Blot analysis of various cells using Creatine Kinase M Polyclonal Antibody

Western blot analysis of lysates from Jurkat, HeLa, HepG2, and HUVEC cells, using M-CK Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using M-CK Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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