CRSP150 Polyclonal Antibody

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Home > Product > Antibody > CRSP150 Polyclonal Antibody

CRSP150 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC-p, IF, ELISA

Product overview

Full product name

CRSP150 Antibody

Code

BT-AP02250

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MED14. AA range:701-750

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED14 (mediator complex subunit 14) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.

Uniprot accession

Human:;Mouse:;

Molecular weight

160607

Gene ID

Human: 9282; Mouse: 26896

Synonyms

MED14; ARC150; CRSP2; CXorf4; DRIP150; EXLM1; RGR1; TRAP170; Mediator of RNA polymerase II transcription subunit 14; Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 t

Research area

Epigenetics and Nuclear Signaling

Target protein

MED14

Recommended dilution

Application	Dilution
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using MED14 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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