CYB5R3 Polyclonal Antibody

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Home > Product > Antibody > CYB5R3 Polyclonal Antibody

CYB5R3 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, ELISA

Product overview

Full product name

CYB5R3 Antibody

Code

BT-AP02340

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CYB5R3. AA range:137-186

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CYB5R3 encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in CYB5R3 cause methemoglobinemias.

Uniprot accession

Human:;Mouse:;

Molecular weight

34235

Gene ID

Human: 1727

Synonyms

CYB5R3; DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1

Research area

Cardiovascular

Target protein

CYB5R3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 40000

Images

Western blot analysis of the lysates from K562 cells using CYB5R3 antibody.

Western Blot analysis of various cells using CYB5R3 Polyclonal Antibody

Western blot analysis of lysates from HepG2, COLO, HUVEC, HT-29, and K562 cells, using CYB5R3 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using CYB5R3 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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