eIF4G Polyclonal Antibody

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Home > Product > Antibody > eIF4G Polyclonal Antibody

eIF4G Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: IHC-p, IF, WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC-p, IF, WB, ELISA

Product overview

Full product name

eIF4G Antibody

Code

BT-AP02900

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human eIF4G. AA range:1074-1123

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The eukaryotic translation initiation factor 4 gamma 1 encoded by EIF4G1 is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Uniprot accession

Human:;Mouse:;

Molecular weight

175535

Gene ID

Human: 1981; Mouse: 208643

Synonyms

EIF4G1; EIF4F; EIF4G; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220

Research area

Epigenetics and Nuclear Signaling

Target protein

EIF4G1

Recommended dilution

Application	Dilution
WB	1: 500 - 2000
IF	1: 200 - 1: 1000
ELISA	1: 5000
IHC	1: 100 - 1: 300

Images

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using eIF4G Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using eIF4G Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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