Galectin-9 Polyclonal Antibody

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Home > Product > Antibody > Galectin-9 Polyclonal Antibody

Galectin-9 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Galectin-9 Antibody

Code

BT-AP03453

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human LEG9. AA range:51-100

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for LGALS9.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

39518

Gene ID

Human: 3965; Mouse: 16859; Rat: 25476

Synonyms

LGALS9; Galectin-9; Gal-9; Ecalectin; Tumor antigen HOM-HD-21

Research area

Cell Biology

Target protein

LGALS9

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western blot analysis of lysates from HepG2 cells, using LEG9 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of HepG2 cells using Galectin-9 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 1) VEC, 2) HepG2 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) (Immunoway:YM3030) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using Galectin-9 Polyclonal Antibody diluted at 1:1000

Immunofluorescence analysis of Hela cell. 1,Galectin-9 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of NIH/3T3 cells, using LEG9 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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