gp91-phox Polyclonal Antibody

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Home > Product > Antibody > gp91-phox Polyclonal Antibody

gp91-phox Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, ELISA

Product overview

Full product name

gp91-phox Antibody

Code

BT-AP03657

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human CYBB. AA range:111-160

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

Uniprot accession

Human:;Mouse:;

Molecular weight

65336

Gene ID

Human: 1536; Mouse: 13058

Synonyms

CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b

Research area

Immunology

Target protein

CYBB

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100

Western Blot analysis of HEPG2 using gp91-phox Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100

Western Blot analysis of K562 cells using gp91-phox Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100

Western blot analysis of lysate from K562 cells, using CYBB Antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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