HDAC5/9 Polyclonal Antibody

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Home > Product > Antibody > HDAC5/9 Polyclonal Antibody

HDAC5/9 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, ELISA

Product overview

Full product name

HDAC5/9 Antibody

Code

BT-AP03925

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HDAC5. AA range:225-274

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase 5 encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for HDAC5.

Uniprot accession

Human:;

Molecular weight

121992

Gene ID

Human: 10014/9734; Mouse: 79221

Synonyms

HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related pro

Research area

Others

Target protein

HDAC5

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HDAC5 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using HDAC5/9 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HepG2 cells, using HDAC5 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of HepG2 cells using HDAC5/9 Polyclonal Antibody diluted at 1:1000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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