HLA-DOBeta Polyclonal Antibody

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Home > Product > Antibody > HLA-DOBeta Polyclonal Antibody

HLA-DOBeta Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, ELISA

Product overview

Full product name

HLA-DObeta Antibody

Code

BT-AP04129

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HLA-DOB. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Uniprot accession

Human:;

Molecular weight

30822

Gene ID

Human: 3112

Synonyms

HLA-DOB; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB

Research area

Others

Target protein

HLA-DOB

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
ELISA	1: 40000

Images

Western blot analysis of HLA-DOB Antibody. The lane on the right is blocked with the HLA-DOB peptide.

Western Blot analysis of various cells using HLA-DOβ Polyclonal Antibody diluted at 1:1000

Western blot analysis of the lysates from HeLa cells using HLA-DOB antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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