HMG-1 Polyclonal Antibody

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Home > Product > Antibody > HMG-1 Polyclonal Antibody

HMG-1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, IP, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, IP, ELISA

Product overview

Full product name

HMG-1 Antibody

Code

BT-AP04142

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HMGB1. AA range:131-180

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

HMGB1 encodes high mobility group protein B1 that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

24894

Gene ID

Human: 3146; Mouse: 1.00862e+008; Rat: 25459

Synonyms

HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Research area

Epigenetics and Nuclear Signaling

Target protein

HMGB1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Western Blot analysis of Jurkat cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus.

Western blot analysis of lysates from Jurkat, HepG2, COLO205, and 293 cells, using HMGB1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of huvec-uv cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus.

Immunofluorescence analysis of Hela cell. 1,HMG-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). p53 Monoclonal Antibody(6C4)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

Western Blot analysis of various cells using HMG-1 Polyclonal Antibody diluted at 1:2000 cells nucleus.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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