HXK I Polyclonal Antibody

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Home > Product > Antibody > HXK I Polyclonal Antibody

HXK I Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

HXK I Antibody

Code

BT-AP04305

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. HK1 encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in HK1 have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

102486

Gene ID

Human: 3098; Rat: 25058

Synonyms

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Research area

Cardiovascular

Target protein

HK1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Western blot analysis of HXK I Polyclonal Antibody, using Hela, MCF7,SW480,293T cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.

Western blot analysis of lysates from HeLa cells, using HXK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using HXK I Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of HeLa cells using HXK I Polyclonal Antibody diluted at 1:1000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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