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Home > Product > Antibody > MADD Polyclonal Antibody

MADD Polyclonal Antibody

MADD Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

MADD Antibody

Code

BT-AP05151

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MADD. AA range:751-800

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. MAP kinase-activating death domain protein encoded by MADD is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.

Uniprot accession

Molecular weight

183303

Gene ID

Human: 8567; Mouse: 228355; Rat: 94193

Synonyms

MADD; DENN; IG20; KIAA0358; MAP kinase-activating death domain protein; Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor

Research area

Cancer

Target protein

MADD

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 5000

Images

Western blot analysis of the lysates from HepG2 cells using MADD antibody.
Western Blot analysis of various cells using MADD Polyclonal Antibody diluted at 1:1000
Western Blot analysis of Jurkat cells using MADD Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of A549 cells, using MADD Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MADD Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO, HeLa, and Jurkat cells, using MADD Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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