MARCH5 Polyclonal Antibody

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Home > Product > Antibody > MARCH5 Polyclonal Antibody

MARCH5 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: IF, WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IF, WB, ELISA

Product overview

Full product name

44260 Antibody

Code

BT-AP05217

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850).

Uniprot accession

Human:;Mouse:;

Molecular weight

31232

Gene ID

Human: 54708; Mouse: 69104

Synonyms

MARCH5; RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protei

Research area

Cell Biology

Target protein

MARCH5

Recommended dilution

Application	Dilution
WB	1: 500 - 2000
ELISA	1: 40000
IF	1: 200 - 1: 1000

Images

Immunofluorescence analysis of Hela cell. 1,MARCH5 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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