MIB1 Polyclonal Antibody

Product overview

Full product name

MIB1 Antibody

Code

BT-AP05407

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Synthetic peptide from human protein at AA range: 901-950

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

MIB1 (mindbomb E3 ubiquitin protein ligase 1) encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Uniprot accession

Human:;Mouse:;

Molecular weight

110136

Gene ID

Human: 57534; Mouse: 225164

Synonyms

MIB1 DIP1 KIAA1323 ZZANK2

Research area

Stem Cells

Target protein

MIB1

Recommended dilution

Application	Dilution
WB	1: 500 - 2000
ELISA	1: 10000 - 20000
IHC-p	100 - 300

Images

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Western Blot analysis of 293t cells using Antibody diluted at 1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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