MMP-13 Polyclonal Antibody

Product overview

Full product name

MMP-13 Antibody

Code

BT-AP05477

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP-13. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

MMP13 encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Collagenase 3 in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.

Uniprot accession

Human:;Mouse:;

Molecular weight

53820

Gene ID

Human: 4322

Synonyms

MMP13; Collagenase 3; Matrix metalloproteinase-13; MMP-13

Research area

Cardiovascular

Target protein

MMP13

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunohistochemical analysis of paraffin-embedded Human Colon cancer. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western Blot analysis of various cells using MMP-13 Polyclonal Antibody diluted at 1:500

Western blot analysis of mouse-brain lysis using MMP-13 antibody. Antibody was diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human Mammary cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Colon cancer. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Mammary cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-13 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1,MMP-13 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Western blot analysis of lysates from LOVO cells, using MMP-13 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Colon cancer. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunofluorescence analysis of HepG2 cells, using MMP-13 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Mammary cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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