MRP-L4 Polyclonal Antibody

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Home > Product > Antibody > MRP-L4 Polyclonal Antibody

MRP-L4 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, ELISA

Product overview

Full product name

MRP-L4 Antibody

Code

BT-AP05587

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPL4. AA range:90-139

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Mammalian mitochondrial ribosomal protein L 4 (39S ribosomal protein L4 mitochondrial) is encoded by nuclear gene MRPL4 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

Uniprot accession

Human:;Mouse:;

Molecular weight

34919

Gene ID

Human: 51073; Mouse: 66163

Synonyms

MRPL4; CDABP0091; CGI-28; 39S ribosomal protein L4; mitochondrial; L4mt; MRP-L4

Research area

Epigenetics and Nuclear Signaling

Target protein

MRPL4

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Western blot analysis of the lysates from HeLa cells using MRPL4 antibody.

Western blot analysis of lysates from COLO cells, using MRPL4 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using MRP-L4 Polyclonal Antibody

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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