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Home > Product > Antibody > MRP-S25 Polyclonal Antibody

MRP-S25 Polyclonal Antibody

MRP-S25 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA

Product overview

Full product name

MRP-S25 Antibody

Code

BT-AP05609

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

28S ribosomal protein S25 mitochondrial (mitochondrial ribosomal protein S25) is encoded by nuclear genes MRPS25 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.

Uniprot accession

Molecular weight

20116

Gene ID

Human: 64432; Mouse: 64658; Rat: 297459

Synonyms

MRPS25; RPMS25; 28S ribosomal protein S25; mitochondrial; MRP-S25; S25mt

Research area

Others

Target protein

MRPS25

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
ELISA 1: 40000

Images

Western Blot analysis of various cells using MRP-S25 Polyclonal Antibody diluted at 1:2000
Western blot analysis of the lysates from HepG2 cells using MRPS25 antibody.
Western blot analysis of lysates from 293 cells, using MRPS25 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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