Myogenin Polyclonal Antibody

Product overview

Full product name

Myogenin Antibody

Code

BT-AP05725

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Myogenin. AA range:50-99

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

25037

Gene ID

Human: 4656; Mouse: 17928; Rat: 29148

Synonyms

MYOG; BHLHC3; MYF4; Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4

Research area

Epigenetics and Nuclear Signaling

Target protein

MYOG

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
ELISA	1: 10000
IHC-p	100 - 300

Images

Western blot analysis of lysate from A549 cells, using Myogenin antibody.

Western Blot analysis of various cells using Myogenin Polyclonal Antibody cells nucleus.

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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