MYPT3 Polyclonal Antibody

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Home > Product > Antibody > MYPT3 Polyclonal Antibody

MYPT3 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, ELISA

Product overview

Full product name

MYPT3 Antibody

Code

BT-AP05742

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human PPP1R16A. AA range:362-411

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by MYPT3 (protein phosphatase 1 regulatory subunit 16A, PPP1R16A) represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while other MYPTs result in PP1c activation after becoming phosphorylated, the encoded protein is phosphorylated by protein kinase A and then inhibits the catalytic activity of PP1c.

Uniprot accession

Human:;Mouse:;

Molecular weight

57811

Gene ID

Human: 84988

Synonyms

PPP1R16A; MYPT3; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3

Research area

Signal Transduction

Target protein

PPP1R16A

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
ELISA	1: 5000

Images

Western blot analysis of PPP1R16A Antibody. The lane on the right is blocked with the PPP1R16A peptide.

Western blot analysis of the lysates from HeLa cells using PPP1R16A antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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