Neddylin Polyclonal Antibody

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Home > Product > Antibody > Neddylin Polyclonal Antibody

Neddylin Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, ELISA

Product overview

Full product name

Neddylin Antibody

Code

BT-AP05872

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human NEDD8. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

NEDD8 is a Protein Coding gene. Among its related pathways are Signaling by GPCR and p53 pathway (Pathway Interaction Database). Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding. An important paralog of this gene is UBA52.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

9072

Gene ID

Human: 4738; Mouse: 18002; Rat: 25490

Synonyms

NEDD8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8

Research area

Cell Biology

Target protein

NEDD8

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 5000

Images

Western Blot analysis of various cells using Neddylin Polyclonal Antibody diluted at 1:1000 cells nucleus.

Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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