p15 Polyclonal Antibody

Product overview

Full product name

p15 Antibody

Code

BT-AP06743

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human p15 INK. AA range:89-138

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CDKN2B lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. CDKN2B encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus cyclin dependent kinase inhibitor 2B functions as a cell growth regulator that controls cell cycle G1 progression. The expression of CDKN2B was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of CDKN2B, which encode distinct proteins, have been reported.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

14722

Gene ID

Human: 1030; Mouse: 12579; Rat: 25164

Synonyms

CDKN2B; MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B

Research area

Cell Biology

Target protein

CDKN2B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p15 INK Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western Blot analysis of various cells using p15 Polyclonal Antibody diluted at 1:500

Immunofluorescence analysis of HeLa cells, using p15 INK Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of lysates from HeLa cells, using p15 INK Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

> Protocol Booklet

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