p27 Polyclonal Antibody

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Home > Product > Antibody > p27 Polyclonal Antibody

p27 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

p27 Antibody

Code

BT-AP06755

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human p27 Kip1. AA range:141-190

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

CDKN1B encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The cyclin dependent kinase inhibitor 1B binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN7).

Uniprot accession

Human:;Mouse:;

Molecular weight

22073

Gene ID

Human: 1027

Synonyms

CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Research area

Cell Biology

Target protein

CDKN1B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Western Blot analysis of 293 cells using p27 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from NIH and 3T3 cells, treated with EGF, using p27 Kip1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of customer's lysis using p27 antibody. Antibody was diluted at 1:1000

Western Blot analysis of various cells using p27 Polyclonal Antibody diluted at 1:1000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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