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Home > Product > Antibody > PEPCK-C Polyclonal Antibody

PEPCK-C Polyclonal Antibody

PEPCK-C Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

PEPCK-C Antibody

Code

BT-AP07056

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Uniprot accession

Molecular weight

69195

Gene ID

Human: 5105; Mouse: 18534; Rat: 362282

Synonyms

PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Phosphoenolpyruvate carboxylase

Research area

Cardiovascular

Target protein

PCK1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 300
ELISA 1: 20000

Images

Western Blot analysis of HeLa, MCF7 cells using PEPCK-C Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100
Western blot analysis of lysate from HeLa cells, using PCK1 Antibody.
Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100

Protocols

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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