PERK Polyclonal Antibody

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Home > Product > Antibody > PERK Polyclonal Antibody

PERK Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

PERK Antibody

Code

BT-AP07066

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human E2AK3. AA range:61-110

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Eukaryotic translation initiation factor 2 alpha kinase 3 encoded by EIF2AK3 phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Eukaryotic translation initiation factor 2 alpha kinase 3 is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in EIF2AK3 are associated with Wolcott-Rallison syndrome.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

125147

Gene ID

Human: 9451; Rat: 29702

Synonyms

EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

Research area

Epigenetics and Nuclear Signaling

Target protein

EIF2AK3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of Hela cell. 1,PERK Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Western blot analysis of PERK Polyclonal Antibody, using Hela, HepG2,SW480,A549 cell, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.

Western blot analysis of lysates from HeLa and HepG2 cells, using E2AK3 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using E2AK3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using PERK Polyclonal Antibody diluted at 1:2000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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