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Home > Product > Antibody > Rad18 Polyclonal Antibody

Rad18 Polyclonal Antibody

Rad18 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat

Applications: WB, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ELISA

Product overview

Full product name

Rad18 Antibody

Code

BT-AP07602

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human RAD18. AA range:301-350

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

RAD18, E3 ubiquitin protein ligase encoded by RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18, E3 ubiquitin protein ligase is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Uniprot accession

Molecular weight

56195

Gene ID

Human: 56852

Synonyms

RAD18; RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73

Research area

Epigenetics and Nuclear Signaling

Target protein

RAD18

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
ELISA 1: 40000

Images

Western blot analysis of the lysates from COLO205 cells using RAD18 antibody.
Western Blot analysis of Jurkat cells using Rad18 Polyclonal Antibody cells nucleus.
Western blot analysis of RAD18 Antibody. The lane on the right is blocked with the RAD18 peptide.
Western Blot analysis of various cells using Rad18 Polyclonal Antibody cells nucleus.

Protocols

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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