Rad52 Polyclonal Antibody

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Home > Product > Antibody > Rad52 Polyclonal Antibody

Rad52 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Rad52 Antibody

Code

BT-AP07612

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human RAD52. AA range:70-119

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

RAD52 homolog, DNA repair protein encoded by RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. RAD52 product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of RAD52 is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of RAD52 have been described, but their full-length nature is not known.

Uniprot accession

Human:;Mouse:;

Molecular weight

46169

Gene ID

Human: 5893; Mouse: 19365

Synonyms

RAD52; DNA repair protein RAD52 homolog

Research area

Epigenetics and Nuclear Signaling

Target protein

RAD52

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Immunofluorescence analysis of COS7 cells, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of 293 cells using Rad52 Polyclonal Antibody cells nucleus.

Western blot analysis of lysates from 293 cells, using RAD52 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Rad52 Polyclonal Antibody cells nucleus.

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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